The Reform of Cinnamic alcohol Dehydrogenase Technology

- Jun 12, 2018 -

Cinnamic alcohol dehydrogenase, as a key enzyme in plant secondary metabolism, especially lignin synthesis, is closely related to plant growth and development and resistance to invasion of pathogens. Lignin is one of the components that make up the cell wall of plants, and has the effect of connecting cells. It has the function of strengthening cell walls and adhesive fibers in plant tissues. Its composition and nature are more complex and have a very strong activity. The relatively high molecular weight substances, which are difficult to hydrolyze under the action of acids, are mainly present in the cells of the lignified plants and strengthen the plant tissues. In addition, lignin also has anti-tumor effects, hepatoprotective and anti-oxidative effects, anti-viral effects, smooth muscle spasmolysis, cAMP phosphodiesterase inhibitory activity, immune enhancement, and promotion of protein and glycogen synthesis.

The most important enzyme for lignin synthesis, currently using RT-PCR and RACE methods to isolate a gene of the CAD gene family from Xiaoshun bamboo shoots, using the cloning technology to produce CAD, but the cost is so high that the price of downstream products is also rising. , to the application of the product has brought great difficulties.

However, recent studies have shown that the use of dehydrogenases to catalyze the reduction of Cinnamic alcohol and its derivatives in NADPH can effectively synthesize CAD. This research result is a major breakthrough in solving the problem of the synthesis of Cinnamic alcohol dehydrogenase, and greatly reduces the manufacturing cost of CAD. This exciting news comes from Hubei Yuancheng Pharmaceutical Co., Ltd. According to reports, the use of this technology for the synthesis of CAD in the performance of the full use of biotechnology and cloned CAD, the downstream products such as lignin synthesis has brought unprecedented profit margins.

Of course, in this process, the role of Cinnamic alcohol is no doubt that it is the basis for the synthesis of CAD, the quality of Cinnamic alcohol directly affects the application of CAD synthesis quality and performance.